H+-Pumping Driven by the Plasma Membrane ATPase in Membrane Vesicles from Radish: Stimulation by Fusicoccin
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چکیده
منابع مشابه
Anion - Sensitive , H + - Pumping ATPase in Membrane Vesicles
H+-pumping ATPases were detected in nicrosomal vesicles of oat (Avena sativa L. var Lang) roots using 114Clmethylamine distribution or quinacrine fluorescent quenching. Methylamine (MeA) accumulation into vesicles and quinacrine quench were specifically dependent on Mg,ATP. Both activities reflected formation of a proton gradient (ApH) (acid inside) as carbonyl cyanide m-chlorophenylhydrazone, ...
متن کاملFusicoccin Activates the Plasma Membrane H+-ATPase by a Mechanism Involving the C-Terminal Inhibitory Domain.
Plasma membrane vesicles isolated from spinach leaves incubated with the fungal toxin fusicoccin showed a twofold increase in ATP hydrolytic activity and a threefold increase in H+ pumping compared to controls. This increase in H+-ATPase activity was largely completed within 4 min of incubation and was not due to de novo synthesis of H+-ATPase as demonstrated by immunoblotting. Incubation with ...
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H(+)-pumping ATPases were detected in microsomal vesicles of oat (Avena sativa L. var Lang) roots using [(14)C]methylamine distribution or quinacrine fluorescent quenching. Methylamine (MeA) accumulation into vesicles and quinacrine quench were specifically dependent on Mg,ATP. Both activities reflected formation of a proton gradient (DeltapH) (acid inside) as carbonyl cyanide m-chlorophenylhyd...
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The present study was conducted to find the effect of three heavy metals, Ag, Hg and Pb on the expression level of a gene encoding plasma membrane H+-ATPase in Aeluropus littoralis. The experiment was laid out in a completely random design with three replications. The expression of the main gene was normalized to the expression of the housekeeping gene actin. Two 259 and 187 bp fragments were a...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1986
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.82.1.121